基于微信平台的医学检验教学的应用

目的通过研究基于微信平台的教学模式来提升医学检验专业学生们的学习能力。方法对某高校医学检验专业学生2014-2016三级学生共170人进行对照实验分析,对照组83人,实验组87人。对照组使用传统教学方式教授医学检验专业,实验组使用基于微信平台的课堂教学方式,教授医学检验专业。教学后,比较两组学生的学习状况和学习成绩。结果实验组在应试结果、课堂参与积极性、课堂学习兴趣、课前是否预习、课后是否复习等指标上分数明显高于对照组,差异具有统计学意义(P<0.05)。且实验组成绩明显高于对照组(P<0.05)。结论设置医学检验专业的高校应积极寻找医学检验课程教学改革的方向和切入点,以微信平台为落脚点的互联网教学模式可以被广泛应用到现在医学检验专业课程教学过程中。     

王晞星辨治大肠癌肝转移经验

介绍王晞星教授辨治大肠癌肝转移的临床经验。认为大肠癌肝转移的根本病机为肝脾两虚、痰瘀毒结;临证以调和肝脾为重,减毒消癥为辅,标本兼顾,攻补兼施;研创特色方药补中调肝汤,和法抗癌,疗效显著。并附验案1则。     

刘晓鹰教授“扶中”外治法治疗儿科疾病临床经验浅析

小儿"脾常不足",脾胃之体成而未全,脾胃之气全而未壮,脾胃不足在儿科疾病发生发展、病机转归中占有相当重要的地位。刘晓鹰教授在继承全国名老中医倪珠英学术思想基础上,提出"扶中"外治法,立法取穴方面强调时时顾护小儿中焦脾胃,采用小儿推拿、扶阳罐(含温推、温灸和温刮痧)、针刺(含腹针、揿针)等外治手段治疗儿科脾、肺、肾等系列疾病,取得满意疗效。     

补肾凉血安胎法治疗肾虚血热型先兆流产合并官腔积液临床研究

目的:研究补肾凉血安胎法治疗肾虚血热型先兆流产合并宫腔积液的效果。方法:选择肾虚血热型先兆流产合并宫腔积液患者160例,按随机数字表法分为对照组和观察组各80例。对照组给予黄体酮联合酚磺乙胺治疗,观察组采用补肾凉血安胎法治疗,连续治疗2周。观察2组临床疗效,比较2组孕酮(P)、雌二醇(E2)、人绒毛膜促性腺激素(HCG),以及中医症候评分。结果:观察组总有效率为85.00%,高于对照组70.00%(P<0.05)。治疗前,2组P、HCG、E2水平比较,差异无统计学意义(P>0.05)。治疗后,2组P、HCG、E2水平较治疗前升高(P<0.05),且观察组P、HCG、E2水平高于对照组(P<0.05)。治疗前,2组各项中医症状评分比较,差异无统计学意义(P>0.05)。治疗后,2组各项中医症状评分较治疗前降低(P<0.05),且观察组各项中医症状评分低于对照组(P<0.05)。结论:采用补肾凉血安胎汤治疗肾虚血热型先兆流产合并宫腔积液患者,不仅能改善患者血液指标,有利于保胎,还能促进宫腔积液的吸收,效果显著,值得临床应用。     

PBL与CBL教学法在留学生实验诊断教学的应用探讨

目的探讨PBL与CBL教学法在留学生实验诊断教学的应用效果。方法将PBL联合案例教学法(CBL)应用于临床医学本科留学生实验诊断教学,与大班传统讲授教学方法(对照组)相比较,通过问卷调查和成绩分析学习效果。结果试验组在激发学习兴趣和培养临床思维能力方面均高于对照组,理论知识的成绩和实习的成绩也是试验组优于对照组,P<0.05。结论PBL与CBL教学联合应用于实验诊断教学中能够有效提高教学质量,并受到学生的认可和喜爱。     

Fertility awareness-based methods of family planning

Fertility awareness-based methods (FABMs) of family planning involve monitoring various signs and symptoms of fertility during the menstrual cycle to identify the “fertile window,” or the days of the cycle when unprotected intercourse is most likely to result in pregnancy. Signs and symptoms include menstrual cycle length, basal body temperature, urinary hormone measurements, and/or cervical fluid and may be used alone or in combination. Fertility signs reflect both physiological changes during the menstrual cycle and the life cycle of the ovum and sperm. Women learn to observe or measure and interpret these signs according to the instructions of their chosen FABM and avoid unprotected intercourse on fertile days. FABMs are appropriate for those who choose to use them, are able and willing to observe one or more fertility signs, and are in relationships that support the use of a coitus-related method such as a condom or abstaining from intercourse on fertile days.     

PBL教学法在临床实习护士查房中的应用

目的探讨PBL教学法在临床实习护士查房中的应用。方法选取2018年1月-2018年11月于本院实习的42例护士作为研究对象,按照查房方法的差异分为对照组(21例)与观察组(21例),对照组采用传统查房法,观察组采用PBL教学法。比较两组护理业务成绩、临床各项评价指标得分情况以及对护理查房业务的掌握程度。结果观察组临床护理业务兴趣、自主学习能力、学习效率、回答问题踊跃程度等各项指标得分高于对照组,差异有统计学意义(P<0.05)。观察组理论考试、实操训练、病历书写等临床各项评价指标得分高于对照组,差异有统计学意义(P<0.05)。观察组对护理查房业务的掌握程度为95.2%,高于对照组的61.9%,差异有统计学意义(P<0.05)。结论在临床护理业务查房中应用教学法,建议采纳PBL教学法,在提高实习护士理论知识、提升业务水平和综合能力方面价值显著。     

厚朴超临界CO_2提取工艺优化及提取物抗氧化活性研究

目的优选超临界CO_2提取厚朴有效成分的工艺并探讨厚朴超临界CO_2提取物的抗氧化活性。方法采用HPLC法测定厚朴超临界CO_2提取物中厚朴酚与和厚朴酚的含量,正交试验优选厚朴超临界CO_2提取工艺,MTT法检测提取物抗氧化活性。结果厚朴酚优化工艺为萃取压力25 MPa,萃取温度55℃,CO_2用量30 kg;和厚朴酚最佳提取工艺压力15 MPa、萃取温度50℃、CO_2用量25 kg。厚朴超临界CO_2提取物具有抗氧化活性,且分离参数不同,抗氧化活性有显著差异。结论在所优选的提取工艺条件下,厚朴酚、和厚朴酚的提取效率较高,重复性较好,工艺稳定可行,提取物具有良好的抗氧化活性。     

Hallux length and deep medial crease in complex clubfeet: Do they recover?

ObjectiveThe shortened hallux and deep medial crease are a significant cosmetic deformity in complex clubfeet. We quantitatively determined the correction of hallux length and deep medial crease following treatment of complex clubfeet.MethodsA chart review of 11 patients (17 feet) with complex clubfeet treated with modified Ponseti method was undertaken. Pretreatment clinical photographs and Pirani scores were compared with those obtained at a recent follow-up to analyze outcomes. Hallux length was matched with 2nd toe and graded similar to Pirani score.ResultsMean patient age at enrollment was 26.8 weeks. Average follow up was 22.6 months. Pre treatment and follow up Pirani score averaged 5.8 and 0.2 respectively. The average number of cast utilized was 7. Incomplete/non correction of hallux was observed in 6 feet (35%), of which 4 suffered an equinus relapse. Deep medial crease corrected in all.ConclusionsThe study describes a clinical method of hallux length quantification in complex clubfeet. Medial crease recovered in all feet. The recovery of hallux length was delayed in some patients and might indicate persistent posteromedial soft tissue contracture/fibrosis in these feet.     

Investigation of factors associated with ABCB5-positive limbal stem cell isolation yields from human donors

PurposeTo identify factors associated with isolation yields of ATP-binding cassette (ABC) superfamily member B5 (ABCB5)-positive limbal stem cells (LSCs) from human cadaveric donor eyes.MethodsWhole eye globes were obtained from the Saving Sight eye bank, Kansas City, MO and the CorneaGen eye bank, Seattle, WA. ABCB5-positive LSCs were sorted by flow cytometry upon anti-ABCB5 monoclonal antibody staining within one week after donor death. The yields of live limbal epithelial cells in their entirety and of isolated pure ABCB5-positive LSC subsets were correlated with variables contained in the eye donors’ medical information.ResultsThe mean isolation yield of live limbal epithelial cells and ABCB5-positive LSCs per donor eye was (340,000 ± 160,000 and 2,608 ± 1,842 respectively, mean ± SD). Stepwise regression analysis showed that cardiac disease-related death was the strongest negative predictor of the ABCB5-positive LSC isolation yield (p = 0.01). While we observed a trend for an age-related decline in the yield of ABCB5-positive LSCs, a statistically significant association could not be established (2% decrease/year, p = 0.11). Additionally, despite a trend for decreased isolation yields of total live limbal epithelial cells isolated from single donors with a longer time between death and tissue processing (p = 0.04), this did not affect the yields of purified ABCB5-positive LSC, which was independent of increasing time between death and tissue processing (p = 0.50).ConclusionsOur study identifies cardiac disease-related death as a donor variable significantly associated with lower ABCB5-positive LSC isolation yields.